Coding
PduD20-GFP
Part:BBa_K562012
Designed by: Frank Sargent Group: iGEM11_Dundee (2011-09-17)
Salty_PduD20-GFP
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 20 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562001), which is itself fused in-frame to GFP. The GFP gene product also carries a C-terminal HA epitope tag. Production of PduD20-GFP has been verified by Western immunoblotting (anti-GFP). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D20-GFP in the Sargent Laboratory, Dundee, UK.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 179
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 926
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 179
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 179
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |